This compound is a specialized 2'-phosphoramidite primarily used in the solid-phase synthesis of modified oligonucleotides, particularly 2'-5'-linked RNA sequences, in molecular biology and genetic research. It serves as a key building block, with the tert-butyldimethylsilyl (TBDMS) group protecting the 3'-hydroxyl, the dimethoxytrityl (DMT) group protecting the 5'-hydroxyl, and the isobutyryl group protecting the N2 position of the guanosine base. The 2'-O-(2-cyanoethyl) phosphoramidite enables controlled and selective coupling at the 2'-position, preventing unwanted side reactions. This reagent is essential for custom modified oligonucleotides applied in synthetic biology, gene editing with non-standard backbones, and research on nucleic acid analogs such as immunostimulatory sequences. Its stability and reactivity make it critical for advanced nucleic acid synthesis platforms.