Primarily used in biochemical research, this compound serves as a modified nucleoside to study nucleic acid structure and function. It is employed in the synthesis of oligonucleotides for probing enzyme mechanisms, particularly those involving DNA and RNA polymerases. Additionally, it is utilized in the development of antiviral and anticancer agents, as its incorporation into nucleic acids can disrupt viral replication and inhibit tumor cell growth. Its fluorine modification enhances stability and binding affinity, making it valuable in diagnostic assays and therapeutic applications.