This chemical is primarily used in the synthesis of oligonucleotides, where it serves as a protected nucleoside. The tert-butyldimethylsilyl (TBDMS) group protects the 2'-hydroxyl position of the ribose sugar, preventing unwanted reactions during the assembly of RNA sequences. The isobutyryl group on the guanine base provides additional protection, ensuring selective reactivity in coupling steps. This compound is particularly valuable in solid-phase synthesis, enabling the production of RNA molecules with high precision and efficiency. It is also employed in research settings for studying RNA structure, function, and interactions, as well as in the development of therapeutic oligonucleotides, such as antisense RNAs and small interfering RNAs (siRNAs).