N4-Methylcytidine is primarily utilized in the field of molecular biology and biochemistry as a modified nucleoside. It plays a significant role in RNA research, particularly in studying RNA modifications and their impact on gene expression and cellular processes. This compound is often employed to investigate the function of methylation in RNA molecules, which can influence RNA stability, translation efficiency, and interaction with proteins. Additionally, it serves as a valuable tool in the synthesis of modified RNA for therapeutic applications, such as the development of mRNA-based vaccines and gene therapies. Its incorporation into RNA sequences helps researchers understand the biological significance of epigenetic modifications and their potential in medical advancements.