Used in biochemical research as a substrate for enzymes involved in nucleotide metabolism, particularly in studies of purine synthesis and salvage pathways. Serves as a building block in RNA synthesis during in vitro transcription processes. Employed in molecular biology assays requiring nucleotide triphosphates, such as PCR with modified templates or nucleic acid labeling techniques. Also utilized in enzymatic assays to measure kinase or phosphatase activity. Plays a role in cellular energy transfer and signaling when studied in metabolic pathway models.