Used in solid-phase oligonucleotide synthesis, particularly for the preparation of modified RNA strands. The 2'-O-TBDMS group provides protection to the ribose 2'-hydroxyl, enhancing stability during synthesis, while the 5'-DMT group allows for stepwise chain elongation and purification via trityl-on purification methods. The phosphoramidite functionality enables efficient coupling to the growing nucleotide chain. This compound is especially valuable in the synthesis of antisense oligonucleotides, siRNA, and other therapeutic RNA molecules where precise modification and high yield are critical. The C2-methyl modification can influence base-pairing stability and nuclease resistance, making it useful in optimizing the biological activity and pharmacokinetic properties of synthetic RNA.