Used as an activating agent in oligonucleotide synthesis, particularly in the formation of phosphodiester bonds. It facilitates the coupling of nucleotide units by enhancing the reactivity of phosphate groups, making it valuable in the solid-phase synthesis of DNA and RNA sequences. Its stability and efficiency support high yields in automated synthesizers, especially in the production of short nucleic acid strands for research and diagnostic applications. Also employed in the preparation of labeled probes and modified oligonucleotides used in molecular biology and genetic testing.