Used in oligonucleotide synthesis, particularly in the preparation of modified RNA strands for research and therapeutic applications. The DMT (dimethoxytrityl) group enables protection of the 5′-hydroxyl during stepwise chain assembly, allowing controlled addition of nucleotides in solid-phase synthesis. The tert-butyldimethylsilyl group protects the 2′-hydroxyl, enhancing stability and selectivity during coupling. The 4-chlorophenyl modification at the O4 position of uridine can influence base-pairing properties and nuclease resistance, making it useful in designing antisense oligonucleotides or siRNA with improved binding affinity and biological stability. The phosphoramidite moiety allows efficient coupling to the growing nucleic acid chain under standard DNA/RNA synthesizer conditions. This compound is especially valuable in the development of modified RNA for gene regulation studies, diagnostics, and RNA-based therapeutics.