P1,P4-Di(adenosine-5′) tetraphosphate ammonium salt is primarily used in biochemical research to study enzyme kinetics and nucleotide metabolism. It serves as a substrate or inhibitor in various enzymatic reactions, particularly those involving ATPases and kinases. This compound is also utilized in the investigation of signaling pathways and energy transfer processes within cells. Additionally, it plays a role in the development of assays to measure enzymatic activity and to understand the mechanisms of nucleotide-dependent enzymes. Its application extends to the study of cellular responses to stress and energy depletion, providing insights into metabolic regulation and cellular physiology.