Used as an intermediate in biochemical pathways related to nucleotide metabolism, particularly in the synthesis of adenosine triphosphate (ATP) analogs and modified RNA molecules. Plays a role in enzymatic reactions involving kinase and phosphorylase enzymes, making it valuable in research on energy transfer processes and antiviral drug development. Commonly applied in studies of metabolic engineering and synthetic biology where labeled or modified nucleotides are required. Also utilized in the production of diagnostic reagents for detecting enzyme activity in clinical and laboratory settings.