Used in biochemical research as an intermediate in pyrimidine metabolism studies. Serves as a reference compound in metabolic pathway analysis, particularly in understanding uracil degradation. Applied in the development and validation of analytical methods for detecting dihydropyrimidine dehydrogenase activity, an enzyme relevant in cancer therapy with 5-fluorouracil. Also utilized in enzymatic assays and diagnostic procedures related to metabolic disorders such as dihydropyrimidine dehydrogenase deficiency.