This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. Its modified structure, including the DMT (dimethoxytrityl) protecting group, aids in the step-by-step assembly of RNA strands during solid-phase synthesis. The acetyl group at N4 and the methyl group at the 2'-O position enhance stability and prevent unwanted side reactions, making it a valuable building block for creating modified RNA molecules. These modified RNAs are often employed in studies involving gene silencing, RNA interference (RNAi), and the development of therapeutic oligonucleotides. Additionally, its use is critical in the production of antisense oligonucleotides and siRNA (small interfering RNA) for targeting specific genetic sequences in research and therapeutic applications.