N4-Acetyl-2'-deoxycytidine is primarily utilized in biochemical research, particularly in the study of DNA modification and repair mechanisms. It serves as a modified nucleoside, aiding in the understanding of how cells process and repair damaged DNA. This compound is also employed in the development of nucleoside analogs for potential therapeutic applications, including antiviral and anticancer drugs. Additionally, it plays a role in epigenetics research, helping to explore the effects of chemical modifications on gene expression and cellular function. Its application extends to the synthesis of oligonucleotides for use in molecular biology techniques, such as PCR and sequencing, to investigate DNA structure and function.