This compound is primarily used in the synthesis of oligonucleotides, particularly in the field of nucleic acid chemistry. It serves as a protected nucleoside intermediate, ensuring selective reactivity during the construction of DNA or RNA sequences. The tert-butyldimethylsilyl (TBDMS) group protects the 3'-hydroxyl, while the dimethoxytrityl (DMT) group safeguards the 5'-hydroxyl, allowing for controlled stepwise assembly in solid-phase synthesis. The acetyl group on the N4 position of cytidine prevents unwanted side reactions during the process. This compound is essential in producing high-purity oligonucleotides for applications such as gene synthesis, PCR primers, and antisense therapeutics. Its use is critical in research and development of genetic engineering, molecular diagnostics, and therapeutic oligonucleotides.