Widely used in oligonucleotide synthesis as a protected nucleoside building block. The dimethoxytrityl (DMT) group acts as a protective group for the 5'-hydroxyl, allowing selective deprotection during solid-phase synthesis. The acetyl group protects the exocyclic amine, preventing unwanted side reactions. Enables precise control over the sequence and length of synthetic DNA strands. Essential in the production of antisense oligonucleotides, primers, and probes for molecular biology applications. Plays a key role in the development of therapeutic oligonucleotides, including those used in gene silencing and mRNA targeting.