This chemical, 5'-O-DMT-2'-O-iBu-N-Bz-Guanosine, is primarily used in the synthesis of RNA oligonucleotides, which are essential in various fields of molecular biology and genetic research. It serves as a protected ribonucleoside building block in solid-phase oligonucleotide synthesis, ensuring the precise assembly of RNA sequences. The protective groups, including DMT (dimethoxytrityl) at the 5'-O position, iBu (isobutyryl) at the 2'-O position, and Bz (benzoyl) on the exocyclic amino group, prevent unwanted reactions during the synthesis process, allowing for the stepwise construction of oligonucleotides with high accuracy. The resulting oligonucleotides are widely employed in applications such as PCR (polymerase chain reaction), gene editing, sequencing, and the development of antisense therapeutics. Additionally, it plays a role in the production of modified nucleic acids for advanced research in drug discovery and diagnostics.