This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside monomer, essential for solid-phase synthesis of RNA sequences. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl, while the TBDMS (tert-butyldimethylsilyl) group protects the 2'-hydroxyl, ensuring selective reactivity during the coupling process. The N-Bz (benzoyl) group protects the adenine base, preventing unwanted side reactions. This compound is crucial for producing high-purity RNA strands, which are widely used in studies involving gene expression, RNA interference, and the development of therapeutic RNA molecules. Its application is particularly significant in the production of synthetic RNA for vaccines, antisense oligonucleotides, and CRISPR-based gene-editing technologies.