This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside intermediate, enabling the controlled assembly of nucleotide sequences. The tert-butyldimethylsilyl (TBDMS) group protects the 2'-hydroxyl position, while the 4-acetyl group safeguards the exocyclic amine, ensuring selective reactivity during chemical reactions. The 5'-O-DMT (dimethoxytrityl) group acts as a temporary protecting group, allowing for stepwise elongation of the oligonucleotide chain. This compound is crucial in solid-phase oligonucleotide synthesis, where precision and protection of functional groups are essential for constructing specific DNA or RNA sequences. Its application is vital in developing primers, probes, and therapeutic oligonucleotides used in diagnostics, gene editing, and antisense technologies.