Used primarily in the synthesis of modified oligonucleotides, this compound plays a crucial role in RNA research and therapeutic development. It serves as a protected nucleoside intermediate, enabling the precise construction of RNA molecules with specific modifications. Its tert-butyldimethylsilyl group provides stability during chemical reactions, while the 2'-O-methyl modification enhances resistance to nuclease degradation. This makes it valuable in creating stable RNA analogs for applications such as antisense oligonucleotides, siRNA, and mRNA therapeutics. Additionally, it is employed in the study of RNA structure and function, aiding in the development of targeted gene silencing and RNA-based drugs.