Used primarily in the synthesis of oligonucleotides, this chemical plays a critical role in the protection of reactive sites during the construction of DNA sequences. Its dimethoxytrityl group serves as a protective moiety for the 5'-hydroxyl group, allowing selective deprotection and elongation in solid-phase synthesis. The N6-methyl modification enhances the stability of the nucleoside, making it valuable in research focused on modified nucleic acids. It is particularly useful in the development of antisense oligonucleotides and other therapeutic nucleic acids, where precise control over the sequence and modifications is essential. Additionally, its application extends to the study of DNA-protein interactions and the exploration of epigenetic modifications, providing insights into gene regulation and expression.