Cytidine sulfate is primarily used in biochemical research and pharmaceutical development. It plays a role in studying nucleic acid metabolism and the synthesis of RNA and DNA. This compound is also utilized in the production of nucleoside analogs, which are critical in antiviral and anticancer therapies. Additionally, it serves as a precursor in the synthesis of other biologically active molecules, contributing to advancements in medicinal chemistry and drug discovery. Its applications extend to cell culture studies, where it aids in understanding cellular processes and metabolic pathways.