This compound is a protected nucleoside phosphoramidite precursor, specifically 5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-5-methyl-2'-deoxycytidine, used in the synthesis of oligonucleotides, particularly in solid-phase DNA synthesis. It serves as a building block for incorporating 5-methyl-2'-deoxycytidine (5-mdC) into DNA sequences, which is essential for studying DNA methylation and epigenetic modifications. The dimethoxytrityl (DMT) group acts as a protective group for the 5'-hydroxyl, allowing selective deprotection and chain elongation during synthesis. The benzoyl group protects the exocyclic N4 amine of 5-methylcytosine, preventing unwanted side reactions. Its application is critical in genetic research, epigenetics, diagnostics, and therapeutic development, enabling the production of custom DNA strands containing 5-mdC for PCR, sequencing, gene editing, and analysis of methylation patterns.