5,6-Dihydrouridine is primarily recognized for its role in the modification of transfer RNA (tRNA). It contributes to the stability and proper functioning of tRNA molecules, which are essential for protein synthesis. By enhancing the structural flexibility of tRNA, it aids in the accurate translation of genetic information into proteins. This modification is particularly important in maintaining the efficiency of cellular processes, especially under stress conditions. Additionally, its presence in tRNA is studied for potential implications in understanding cellular responses to environmental changes and in the development of therapeutic strategies targeting RNA modifications.