N4-Benzoyl-5'-O-tert-butyldimethylsilyl-2'-deoxycytidine is primarily used in the synthesis of modified nucleosides and nucleotides. It serves as a key intermediate in the preparation of oligonucleotides, which are essential for research in molecular biology and genetic engineering. The tert-butyldimethylsilyl (TBDMS) group protects the 5'-hydroxyl during chemical reactions, ensuring selective modification of other functional groups. The benzoyl group at the N4 position stabilizes the cytidine base, preventing unwanted side reactions. This compound is particularly valuable in the automated synthesis of DNA sequences, enabling the production of custom oligonucleotides for applications such as PCR, gene editing, and antisense therapy. Its use ensures high yield and purity in the construction of complex nucleic acid structures.