Used as a key intermediate in the synthesis of oligonucleotides through solid-phase phosphoramidite chemistry. Its primary role is to protect the 3′ and 5′ hydroxyl groups of thymidine during DNA synthesis, allowing selective coupling at the desired position. The tert-butyldimethylsilyl (TBDMS) groups are stable under acidic and basic conditions but can be selectively removed using fluoride ions, making them ideal for stepwise assembly of DNA strands. This compound is especially useful in the production of antisense oligonucleotides, primers for PCR, and other nucleic acid-based therapeutics where precise control over sequence and purity is essential.