2'-Deoxyinosine is primarily used in molecular biology and biochemistry research as a nucleoside analog. It is incorporated into DNA during replication, where it can pair with multiple bases, making it useful for studying mutagenesis and DNA repair mechanisms. Additionally, it is utilized in the synthesis of oligonucleotides for various applications, including PCR and sequencing. In therapeutic contexts, 2'-Deoxyinosine has been explored for its potential antiviral properties, particularly in inhibiting viral replication by disrupting nucleic acid synthesis. It is also employed in the development of prodrugs and as a component in diagnostic assays to detect specific genetic mutations.