2',3'-O-Isopropylideneguanosine is primarily used in the field of organic synthesis and biochemical research. It serves as a protected form of guanosine, where the isopropylidene group shields the 2' and 3' hydroxyl positions, preventing unwanted reactions during complex synthetic processes. This protection is crucial in the synthesis of nucleosides, nucleotides, and oligonucleotides, where selective reactivity is required. Additionally, it is utilized in the development of modified RNA and DNA analogs, aiding in the study of nucleic acid structure, function, and interactions. Its application extends to the production of antiviral and anticancer agents, where modified nucleosides play a key role in therapeutic strategies.