This compound is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a key building block in the solid-phase synthesis of modified RNA strands. The 2'-O-(2-methoxyethyl) modification enhances the stability and binding affinity of the resulting RNA, making it valuable for applications in antisense therapy and RNA interference (RNAi). The DMT (dimethoxytrityl) group protects the 5'-hydroxyl during synthesis, allowing for controlled elongation of the oligonucleotide chain. The benzoyl group protects the exocyclic amine, ensuring selective reactivity. This phosphoramidite is essential for producing RNA analogs with improved pharmacokinetic properties, which are critical for therapeutic and diagnostic purposes.