This compound is primarily used in the synthesis of oligonucleotides, particularly in automated solid-phase DNA synthesis. It serves as a building block for the controlled assembly of DNA sequences, enabling the creation of custom DNA strands for research, diagnostics, and therapeutic applications. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl, ensuring selective coupling during synthesis, while the benzoyl group protects the exocyclic amine, preventing unwanted side reactions. Its phosphoramidite functionality allows for efficient coupling with the growing DNA chain on a solid support. This chemical is essential in producing high-quality DNA for applications such as gene editing, PCR primers, and antisense oligonucleotides.