This compound is primarily used in the synthesis of oligonucleotides, particularly in the field of nucleic acid research and therapeutic development. Its role as a phosphoramidite makes it a key building block in solid-phase oligonucleotide synthesis, enabling the creation of modified DNA or RNA sequences. The 2'-fluoro modification enhances the stability and binding affinity of the resulting oligonucleotides, making it valuable for applications such as antisense therapy, siRNA, and diagnostic probes. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl during synthesis, ensuring controlled and precise chain elongation. This chemical is essential in the development of advanced genetic tools and therapeutic agents targeting specific RNA sequences.