This compound, 5'-O-DMT-N6-methyl-2'-deoxyadenosine 3'-CE phosphoramidite, is primarily used in the synthesis of oligonucleotides, particularly in the field of genetic research and molecular biology. It serves as a building block for incorporating N6-methyl-2'-deoxyadenosine into DNA sequences during solid-phase synthesis, enabling the creation of custom strands with enhanced stability for applications such as PCR, gene editing, and diagnostic assays.

The presence of the DMT (dimethoxytrityl) group protects the 5'-hydroxyl during synthesis, while the phosphoramidite moiety with cyanoethyl (CE) protection allows for efficient coupling with other nucleotides. The N6-methyl modification on the adenine base improves the chemical properties and stability of the resulting oligonucleotides.

Its role is critical in producing high-purity modified DNA for studies in genomics, drug development, and therapeutic interventions.