Used primarily in the synthesis of oligonucleotides, it acts as a key reagent in the phosphoramidite method for DNA and RNA sequencing. This chemical facilitates the formation of phosphodiester bonds between nucleotides, crucial for constructing genetic material in laboratory settings. It is also employed in the development of therapeutic agents, including antisense oligonucleotides, which are designed to target specific RNA sequences to treat genetic disorders. Additionally, its role extends to the production of modified nucleic acids for research in molecular biology and genetic engineering.