Used in solid-phase oligonucleotide synthesis, this compound enables the incorporation of a levulinoyl-protected thymidine moiety at the 5′-end of DNA strands. The levulinoyl group acts as a temporary protecting group that can be selectively removed under mild conditions, allowing for site-specific modifications during the synthesis of complex oligonucleotides. Its phosphoramidite functionality ensures efficient coupling to the growing DNA chain on automated synthesizers. This makes it valuable in the production of modified primers, probes, and functional nucleic acids used in molecular biology, diagnostics, and pharmaceutical development.