Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the site-specific incorporation of 2′-O-methyl RNA modifications. These modifications enhance nuclease resistance and binding affinity in antisense oligonucleotides, siRNA, and diagnostic probes. The 2'-OMe modification improves stability and reduces off-target effects in therapeutic nucleic acid applications. The beta-cyanoethyl group ensures efficient coupling and protects the phosphate moiety during synthesis, allowing high-yield, automated production of modified RNA sequences for research and drug development.