Used in the solid-phase synthesis of RNA oligonucleotides, particularly in the preparation of modified RNA strands for research and therapeutic applications. The 2'-O-TBDMS group provides protection to the 2'-hydroxyl, ensuring regioselective coupling during phosphoramidite chemistry, while the 5'-DMT group allows for monitoring of coupling efficiency and purification. The 3-methyluridine modification enhances nuclease resistance and can influence base-pairing stability, making it valuable in the development of antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics. The phosphoramidite functionality enables automated synthesis, facilitating the incorporation of this modified nucleoside into precise positions within RNA sequences.