Used in the solid-phase synthesis of oligonucleotides, particularly in the preparation of modified RNA strands. The compound enables the incorporation of a 1-methylguanosine base with specific protecting groups that ensure correct phosphodiester bond formation during automated synthesis. The 2'-O-tert-butyldimethylsilyl group protects the 2' hydroxyl, preventing unwanted side reactions, while the 5'-O-DMT group allows for stepwise chain elongation and purification via trityl-on strategies. The CE phosphoramidite moiety ensures efficient coupling to the growing nucleic acid chain. Commonly applied in the production of mRNA analogs, antisense oligonucleotides, and other functional RNAs where precise base modifications are required for stability, translation efficiency, or biological activity.