Used in solid-phase oligonucleotide synthesis, this phosphoramidite enables incorporation of deoxynebularine, a neutral nucleoside analog lacking the 2-amino group of guanosine. Its primary application is in the study of DNA-protein interactions and base-pairing fidelity, as deoxynebularine forms weak hydrogen bonds, allowing researchers to probe the role of specific hydrogen bonding in DNA recognition and stability. The DMT (dimethoxytrityl) group provides 5′-hydroxyl protection during synthesis, while the phosphoramidite moiety allows for automated coupling on DNA synthesizers. Commonly used in creating degenerate sequences, mutagenesis studies, and functional genomics tools where reduced base-pairing specificity is desired.