Used in solid-phase oligonucleotide synthesis, particularly for the preparation of RNA strands with high efficiency and fidelity. The 2'-O-TBDMS group protects the 2'-hydroxyl of guanosine, preventing unwanted side reactions and ensuring correct phosphodiester bond formation during chain elongation. The 5'-DMT (dimethoxytrityl) group enables real-time monitoring of coupling efficiency and facilitates purification by reversed-phase chromatography. The phosphoramidite moiety allows for automated coupling under mild conditions, making it ideal for DNA/RNA synthesizers. The tert-butylphenoxyacetyl group protects the exocyclic amine of guanine, offering stability during synthesis and easy removal during final deprotection. This compound is essential in the production of siRNA, antisense oligonucleotides, and other therapeutic RNA molecules requiring precise chemical modification and high purity.