Used in solid-phase oligonucleotide synthesis, this phosphoramidite enables the site-specific incorporation of fluorine-labeled nucleotides into DNA strands. The fluorine atom acts as a probe for NMR studies, allowing detailed analysis of nucleic acid structure and dynamics. It is particularly valuable in developing diagnostic probes and studying hybridization behavior in real time. Its compatibility with automated synthesizers makes it suitable for high-throughput production of modified oligonucleotides used in research and therapeutic applications such as antisense technology and molecular imaging.