Used in solid-phase oligonucleotide synthesis, this phosphoramidite enables the incorporation of a cleavable ethylene glycol linker into DNA or RNA strands. The DMT (dimethoxytrityl) group allows for reversible protection during synthesis, facilitating stepwise addition of nucleotides. The ethylene glycol spacer enhances solubility and reduces non-specific binding, making it valuable in diagnostic probes, antisense oligonucleotides, and molecular beacons. After synthesis, the CE (cyanoethyl) group is removed, and the linker can be cleaved under mild conditions, useful in array-based applications and automated DNA synthesis platforms.