Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the incorporation of thymidine residues into DNA strands with high efficiency and specificity. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl during synthesis, allowing for stepwise addition and real-time monitoring of chain elongation through trityl cation release. The 2'-CE (cyanoethyl) group stabilizes the phosphate during coupling and is later removed under mild base conditions. Its primary application is in the production of synthetic DNA for research, diagnostics, and therapeutic oligonucleotides, including antisense agents, siRNA, and PCR primers. The compound’s design ensures high coupling yields and compatibility with automated synthesizers, making it essential in modern molecular biology and drug development workflows.