Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the incorporation of modified guanosine into RNA strands. The 3'-deoxy feature prevents unwanted extension at the 3' position, ensuring controlled chain elongation. The N2-DMF group protects the guanine base, reducing side reactions during coupling, while the 5'-O-DMT group allows for efficient monitoring and purification of the growing oligonucleotide chain. The 2'-CE (cyanoethyl) group stabilizes the phosphate during synthesis and is later removed under standard deprotection conditions. This compound is especially valuable in the production of antisense oligonucleotides, siRNA, and other therapeutic RNA molecules where precise modifications enhance stability, specificity, and biological activity.