Used in solid-phase oligonucleotide synthesis to incorporate modified nucleosides into DNA strands. This modification enhances resistance to nuclease degradation and improves the stability of antisense oligonucleotides and siRNA. Commonly applied in the development of therapeutic nucleic acids, diagnostic probes, and research tools where increased binding affinity and biological stability are required. The phosphoramidite group enables efficient coupling during automated synthesis, making it valuable in producing modified DNA for biomedical and biotechnological applications.