Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the incorporation of modified guanosine residues into RNA strands. The DMT group provides temporary 5'-end protection, allowing stepwise chain elongation with high efficiency. The TBDMS group selectively protects the 2'-hydroxyl, ensuring ribose stability during synthesis while being removable under mild conditions post-assembly. The N2-acetyl group prevents unwanted side reactions at the guanine base during coupling and deprotection steps. Its phosphoramidite functionality ensures rapid and high-yield coupling to the growing oligonucleotide chain, making it essential for synthesizing complex RNA sequences used in research, diagnostics, and therapeutic applications such as antisense oligonucleotides and siRNA.