Used in solid-phase oligonucleotide synthesis, this phosphoramidite derivative enables the site-specific incorporation of a photoreactive uridine analog into DNA strands. The 5-(3-methacryloyl) group acts as a crosslinking agent upon UV irradiation, allowing for the study of nucleic acid-protein interactions or for immobilizing oligonucleotides onto surfaces. The DMT (dimethoxytrityl) group provides temporary 5'-end protection during synthesis, ensuring stepwise chain elongation, while the phosphoramidite moiety facilitates efficient coupling to the growing oligonucleotide chain. Commonly applied in the preparation of functionalized DNA probes, microarrays, and photo-crosslinking studies in molecular biology.