Used in solid-phase oligonucleotide synthesis, this phosphoramidite building block enables the incorporation of modified guanosine into DNA strands. The DMT group provides protection at the 5'-end, allowing stepwise chain elongation with high efficiency and yield. The N2-(4-isopropylphenoxyacetyl) modification stabilizes the base during synthesis and can influence hybridization properties in the final oligo. The 3'-CE phosphoramidite functionality ensures proper coupling to the growing nucleic acid chain on automated synthesizers. Commonly applied in the production of antisense oligonucleotides, primers, and probes where modified bases enhance nuclease resistance or target binding affinity.