Used as a key reagent in oligonucleotide synthesis, particularly in the preparation of phosphoramidite building blocks for DNA and RNA. It enables efficient coupling reactions by acting as a phosphitylating agent, facilitating the formation of phosphite triester linkages in automated synthesizers. Its bulky isopropyl groups enhance selectivity and reduce side reactions, improving coupling efficiency and overall yield in nucleic acid assembly. Commonly applied in the production of antisense oligonucleotides, primers, probes, and modified nucleic acids for research, diagnostics, and therapeutic development.