Used in solid-phase oligonucleotide synthesis, this phosphoramidite derivative enables the site-specific incorporation of amino-modified thymidine residues into DNA strands. The 5'-amino group, protected by the MMT (monomethoxytrityl) group, allows for selective deprotection under mild acidic conditions, facilitating downstream conjugation with fluorescent dyes, biotin, or other functional labels after synthesis. The 3'-CE (cyanoethyl) phosphoramidite group ensures efficient coupling to the growing DNA chain on automated synthesizers. Commonly applied in the production of labeled probes, primers, and molecular beacons for diagnostic assays, real-time PCR, and nucleic acid detection technologies. Its controlled reactivity and compatibility with standard synthesis protocols make it valuable in developing modified oligonucleotides for research and therapeutic applications.