3-Methyluridine is primarily utilized in the field of biochemistry and molecular biology for research purposes. It serves as a modified nucleoside, aiding in the study of RNA structure and function. This compound is often used to investigate the role of methylation in RNA molecules, which is crucial for understanding gene expression and regulation. Additionally, 3-Methyluridine is employed in the synthesis of modified RNA sequences, helping scientists explore the impact of methylation on RNA stability, translation, and interaction with proteins. Its applications also extend to the development of therapeutic agents, particularly in the design of RNA-based drugs that target specific genetic disorders or diseases.