N6-Methyl-2'-deoxyadenosine plays a significant role in the field of epigenetics and molecular biology. It is primarily used in research to study DNA methylation processes, which are crucial for understanding gene expression regulation. This modified nucleoside helps scientists investigate how methylation at specific sites on DNA can influence cellular functions, including development, aging, and disease progression. Additionally, it is utilized in the development of diagnostic tools and therapeutic strategies for conditions linked to aberrant DNA methylation, such as cancer and neurological disorders. Its application extends to the synthesis of oligonucleotides for probing methylation patterns and studying the interactions between DNA and proteins.